Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : OMICS Publishing Group
Résumé : Coastal tropical shrimp farming may impact the adjacent ecosystems through the release of large quantities of effluents rich in nutrients. In New Caledonia, mangroves are considered as a natural biofilter to reduce impacts on the surrounding World Heritage listed lagoon. Our main objective was to understand the influence of effluent discharge on the biogeochemistry of mangrove sediments. A monitoring of the physico-chemical parameters of mangrove sediments was carried out during a whole year, including active and non active periods of the farm. The parameters studied were: i) benthic primary production (Chl-a concentrations), ii) physico-chemical parameters of sediments (redox potential, pH, salinity, TOC, TN, TS, δ13C and δ15N), iii) concentrations of dissolved nitrogen, iron and phosphorus. A mangrove developing in the same physiographic conditions, presenting the same zonation, and free of anthropogenic input was used as reference. The concentration of benthic Chl-a measured at sediment surface in the effluent receiving mangrove was twice to three times that measured in the control zone whatever the season. We thus suggest that nutrients inputs significantly increased the phytobenthic production in the effluent receiving mangrove during the whole year, even after the cessation of discharges and because of natural seasonal dynamic of phytobenthos. Although the flow of surface OM was increased, the OM content at depth was not higher than in the control mangrove. However, the contribution of mangrove detritus to the sedimentary organic pool was higher probably as a result of higher density and much greater individual size of the mangrove trees. Unlike the control mangrove sediment, the effluent receiving mangrove sediment was not stratified, redox potential values were high and presence of Fe3+ was detected down to 50 cm depth, probably as a result of a larger root system, allowing a better sediment oxygenation and accentuated OM decomposition processes, and thus limiting ecosystem saturation.
Journal of Aquaculture Research & Development (21559546) (OMICS Publishing Group), 2014-07-03 , Vol. 5 , N. 5 , P. 1-13

Droits : 2014 Marchand C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://archimer.ifremer.fr/doc/00251/36253/34801.pdf
DOI:10.4172/2155-9546.1000262
http://archimer.ifremer.fr/doc/00251/36253/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
OBJECTIVES: The aims of the study were to assess in patients with advanced HIV disease receiving antiretroviral therapy (ART) intensification with enfuvirtide (i) resistance at virological failure (VF), (ii) impact of baseline tropism on immunovirological response, and (iii) HIV-1 DNA tropism evolution during ART. METHODS: The ANRS 130 APOLLO randomized trial evaluated in naive patients the immunovirological impact of standard ART without (control arm) or with enfuvirtide. Tropism was determined on RNA and DNA by V3-loop sequencing interpreted using the Geno2Pheno algorithm. RESULTS: At baseline the median CD4 cell count was 30 cells/mm(3). Among the 170 patients assessable in this virological substudy, HIV-1 RNA tropism was as follows: 60% of viruses were R5 and 40% were R5X4/X4. HIV-1 DNA tropism was as follows: 54% were R5 and 46% were R5X4/X4. At week 24, 39% and 49% of patients experienced VF in the enfuvirtide and control arms, respectively. In the enfuvirtide arm, only resistance-associated mutations to enfuvirtide were detected. In the control arm, two patients displayed drug-resistant viruses at the time of VF. No impact of baseline tropism was observed on immunovirological response, regardless of the study arm. Among the 25 patients experiencing DNA tropism switch between baseline and week 24, 16 (64%) switched from R5 to R5X4/X4. These latter were mostly successfully suppressed patients receiving enfuvirtide and exhibiting poorer immunological response. CONCLUSIONS: Baseline RNA tropism had no impact on the immunovirological response. Drug resistance mutations were only detected for the fusion inhibitor. Finally, the mechanism of replenishment of the viral cellular reservoir with X4 viruses observed needs to be further analysed.
ISSN: 0305-7453

hal-00875130
https://hal.archives-ouvertes.fr/hal-00875130
DOI : 10.1093/jac/dks455
PUBMED : 23152480

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Forest ecosystems have integral roles in climate stability, biodiversity and economic development. Soil stewardship is essential for sustainable forest management. Organic matter (OM) removal and soil compaction are key disturbances associated with forest harvesting, but their impacts on forest ecosystems are not well understood. Because microbiological processes regulate soil ecology and biogeochemistry, microbial community structure might serve as indicator of forest ecosystem status, revealing changes in nutrient and energy flow patterns before they have irreversible effects on long-term soil productivity. We applied massively parallel pyrosequencing of over 4.6 million ribosomal marker sequences to assess the impact of OM removal and soil compaction on bacterial and fungal communities in a field experiment replicated at six forest sites in British Columbia, Canada. More than a decade after harvesting, diversity and structure of soil bacterial and fungal communities remained significantly altered by harvesting disturbances, with individual taxonomic groups responding differentially to varied levels of the disturbances. Plant symbionts, like ectomycorrhizal fungi, and saprobic taxa, such as ascomycetes and actinomycetes, were among the most sensitive to harvesting disturbances. Given their significant ecological roles in forest development, the fate of these taxa might be critical for sustainability of forest ecosystems. Although abundant bacterial populations were ubiquitous, abundant fungal populations often revealed a patchy distribution, consistent with their higher sensitivity to the examined soil disturbances. These results establish a comprehensive inventory of bacterial and fungal community composition in northern coniferous forests and demonstrate the long-term response of their structure to key disturbances associated with forest harvesting. The ISME Journal (2012) 6, 2199-2218; doi:10.1038/ismej.2012.84; published online 2 August 2012
ISSN: 1751-7362

hal-01546179
https://hal.archives-ouvertes.fr/hal-01546179
DOI : 10.1038/ismej.2012.84

Éditeur(s) : HAL CCSD Oldendorf/Luhe
Résumé : International audience
Non-calcifying photosynthetic anthozoans have emerged as a group that may thrive under high carbon dioxide partial pressure (pCO(2)) conditions via increased productivity. However, the physiological mechanisms underlying this potential success are unclear. Here we investigated the impact of high pCO(2) on the dissolved inorganic carbon (DIC) use in the temperate sea anemone Anemonia viridis. We assessed the impacts of long-term exposure to high pCO(2), i.e. sampling in situ natural CO2 vents (Vulcano, Italy), and short-term exposure, i.e. during a 3 wk controlled laboratory experiment. We focused on photo-physiological parameters (net photosynthesis rates, chlorophyll a content and Symbiodinium density) and on carbonic anhydrase (CA) activity, an enzyme involved in the energy-demanding process of DIC absorption. Long-term exposure to high pCO(2) had no impact on Symbiodinium density and chlorophyll a content. In contrst, short-term exposure to high pCO(2) induced a significant reduction in Symbiodinium density, which together with unchanged net photosynthesis resulted in the increase of Symbiodinium productivity per cell. Finally, in both in situ long-term and laboratory short-term exposure to high pCO(2), we observed a significant decrease in the CA activity of sea anemones, suggesting a change in DIC use (i.e. from an HCO3- to a CO2 user). This change could enable a shift in the energy budget that may increase the ability of non-calcifying photosynthetic anthozoans to cope with ocean acidification.
ISSN: 0171-8630

hal-01546163
https://hal.archives-ouvertes.fr/hal-01546163
DOI : 10.3354/meps11916

Éditeur(s) : HAL CCSD European Geosciences Union (EGU)
Résumé : International audience
Pollen and clay mineralogical analyses of a Holocene sequence from Sebkha Boujmel (southern Tunisia) traces the climatic and environmental dynamics in the lower arid bioclimatic zone over the last 8000 years. During the Mid- to Late Holocene transition, between 8 and 3 ka, a succession of five wet/dry oscillations is recorded. An intense arid event occurs between 5.7 and 4.6 ka. This episode marks the onset of a long-term aridification trend with a progressive retreat of Mediterranean woody xerophytic vegetation and of grass steppes. It ends with the establishment of pre-desert ecosystems around 3 ka. The millennial-scale climate change recorded in the data from Sebkha Boujmel is consistent with records from the south and east Mediterranean, as well as with climatic records from the desert region for the end of the African Humid Period (AHP). Eight centennial climatic events are recorded at Sebkha Boujmel and these are contemporary with those recorded in the Mediterranean and in the Sahara. They indicate a clear coupling between the southern Mediterranean and the Sahara before 3 ka. The event at 4.2 ka is not evidenced and the link between events recorded in Sebkha Boujmel and the North Atlantic Cooling events is clearer from 3 ka onwards. These variations indicate the importance of climatic determinism in the structuring of landscapes, with the establishment of the arid climatic conditions of the Late Holocene. It is only from 3 ka onwards that the dynamic of plant associations is modified by both human activity and climatic variability. The climatic episodes identified during the historic period indicate strong regionalisation related to the differential impact of the North Atlantic Oscillation (NAO) and the Mediterranean Oscillation (MO) on the Mediterranean basin. The local human impact on regional ecosystems is recorded in the form of episodes of intensification of pastoral and/or agricultural activities. The development of olive production and of several taxa associated with agriculture attest to increasing sedentism among human populations during Classical Antiquity. The significant increase in Artemisia (wormwood) between 1.1 and 0.8 ka (850–1150 AD) is linked to intensive pastoral activity, associated with heightened interannual and/or seasonal climatic instability. A complete re-shaping of the landscape is recorded during the 20th century. The remarkable expansion of the olive tree, and the deterioration of regional ecosystems with the spread of desert species, is linked to recent local socio-economic changes in Tunisia.
ISSN: 1814-9324

hal-01319967
https://hal.archives-ouvertes.fr/hal-01319967
https://hal.archives-ouvertes.fr/hal-01319967/document
https://hal.archives-ouvertes.fr/hal-01319967/file/cp-12-1339-2016.pdf
DOI : 10.5194/cp-12-1339-2016

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : 6 pages
International audience
Large impacts provide a mechanism for resurfacing planets through mixing near-surface rocks with deeper material. Central peaks are formed from the dynamic uplift of rocks during crater formation. As crater size increases, central peaks transition to peak rings. Without samples, debate surrounds the mechanics of peak-ring formation and their depth of origin. Chicxulub is the only known impact structure on Earth with an unequivocal peak ring, but it is buried and only accessible through drilling. Expedition 364 sampled the Chicxulub peak ring, which we found was formed from uplifted, fractured, shocked, felsic basement rocks. The peak-ring rocks are cross-cut by dikes and shear zones and have an unusually low density and seismic velocity. Large impacts therefore generate vertical fluxes and increase porosity in planetary crust.
ISSN: 0036-8075

hal-01399386
https://hal.archives-ouvertes.fr/hal-01399386
PUBMED : 27856906
DOI : 10.1126/science.aah6561

Éditeur(s) : HAL CCSD Geological Society of America
Résumé : orogenic wedges
Interaction between surface and tectonic processes plays a key role in the structural evolution, kinematics, and exhumation of rocks in orogenic wedges. The deformation patterns observed in analog models show that strain partitioning has a strong impact on the vertical component of displacement of tectonic units, which in return favors erosion in domains of important uplift. Partitioning is controlled by tectonic processes and by climate-dependent surface processes, including erosion and sedimentation. The effects of partitioning include localization of deformed domains, exhumation above areas of deep underplating, and steady-state maintenance of wedges for long time periods. Simple models illustrate well how the morphostructural evolution of mountain belts is determined by these complex interactions.
ISSN: 1052-5173

hal-00558931
https://hal.archives-ouvertes.fr/hal-00558931
DOI : 10.1130/GSATG48A.1

Éditeur(s) : HAL CCSD Ecological Society of America
Résumé : Herbivory is viewed as a major driver of plant evolution and the mostimportant energy pathway from plants to higher trophic levels. Therefore, understandingpatterns of herbivory on plants remains a key focus in evolution and ecology. Theevolutionary impacts of leaf herbivory include altering plant fitness, local adaptation, theevolution of defenses, and the diversification of plants as well as natural enemies. Leafherbivory also impacts ecological processes such as plant productivity, communitycomposition, and ecosystem nutrient cycling. Understanding the impact of herbivory onthese ecological and evolutionary processes requires species-specific, as opposed tocommunity-level, measures of herbivory. In addition, species-specific data enables the use ofmodern comparative methods to account for phylogenetic non-independence. Althoughhundreds of studies have measured natural rates of leaf consumption, we are unaware of anyaccessible compilation of these data. We created such a data set to provide the raw dataneeded to test general hypotheses relating to plant–herbivore interactions and to test theinfluence of biotic and abiotic factors on herbivory rates across large spatial scales. A largerepository will make this endeavor more efficient and robust. In total, we compiled 2641population-level measures for either annual or daily rates of leaf herbivory across 1145 speciesof vascular plants collected from 189 studies. All damage measures represent naturaloccurrences of herbivory that span numerous angiosperm, gymnosperm, and fern species. Toenable researchers to explore the causes of variation in herbivory and how these mightinteract, we added information about the study sites including: geolocation, climateclassification, habitat descriptions (e.g., seashore, grassland, forest, agricultural fields), andplant trait information concerning growth form and duration (e.g., annual vs. perennial). Wealso included extensive details of the methodology used to measure leaf damage, includingseasons and months of sampling, age of leaves, and the method used to estimate percentagearea missing. We anticipate that these data will make it possible to test important hypothesesin the plant–herbivore literature, including the plant apparency hypothesis, the latitudinalherbivorydefense hypothesis, the resource availability hypothesis, and the macroevolutionaryescalation of defense hypothesis.
ISSN: 0012-9658

hal-01601627
https://hal.archives-ouvertes.fr/hal-01601627
DOI : 10.1890/13-1741.1
PRODINRA : 397136
PUBMED : 24870043

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
1. The Before-After Control-Impact Paired Series (BACIPS) design distinguishes natural spatial and temporalvariability from variation induced by an environmental impact (or intervention) of interest. BACIPS is a powerfultool to derive inferences about interventions when classic experimental approaches (e.g. which rely on spatialreplicates and random assignment of treatments) are not feasible or desirable. Previously applied BACIPSdesigns generally assume that effects are sudden, constant and long-lived: that is, that systems exhibit ‘stepchanges’in response to interventions. However, complex ecological interactions or gradual interventions maycreate delayed and/or progressive responses, potentially impeding the reliability of classic (step-change) analyses.2. We propose a novel approach, the Progressive-Change BACIPS, which generalizes and expands the scope ofBACIPS analyses.We evaluate the relative performance of this approach using both simulated and real data thatexhibit step-change, linear, asymptotic and sigmoid responses following an intervention.We quantify the statisticalpower and accuracy of the Progressive-Change BACIPS under varying initial population densities, intensityof spatial sampling, effect sizes and number of sampling dates After the intervention.3. We show that Progressive-Change BACIPS identified the correct model among the set of candidate modelsunder most conditions and led to accurate estimates of the parameters that were used to generate the simulateddata. When data were sparse, and the dynamics complex, simpler (more parsimonious) models were favouredover the more complex models that actually generated the simulated data. Application of the Progressive-Change BACIPS to existing data sets from the literature led to strong support for specific models (over alternatives)and led to more specific inferences than possible under the classic BACIPS approach.4. The Progressive-Change BACIPS proposed here is more flexible than the original BACIPS formulationbecause the data are used to inform the form of the final model, rather than having the form of the modelimposed on the data. This leads to better estimates of the effects of environmental impacts and the time-scalesover which they operate. As a result, the Progressive-Change BACIPS should be applicable to a wide range ofstudies and should help improve investigation of time-dependent effects. R code to perform Progressive-ChangeBACIPS analysis is provided.
ISSN: 2041-210X

hal-01416668
https://hal-univ-perp.archives-ouvertes.fr/hal-01416668
DOI : 10.1111/2041-210X.12655

Éditeur(s) : HAL CCSD Impact journals
Résumé : International audience
Interleukin (IL)-33 has been recently reported to be strongly pro-fibrogenic in various models of liver disease. Our aim was to study the role of endogenous IL-33 in a diet-induced model of steatohepatitis. IL-33 deficient mice and wild type (WT) littermates received a high-fat diet (HFD), or a standard diet for 12 weeks. The HFD-induced steatohepatitis was associated with an upregulation of IL-33 transcripts and protein. An insulin tolerance test revealed lower systemic insulin sensitivity in IL33-/-HFD mice than in WT-HFD mice. Nevertheless, IL-33 deficiency did not affect the severity of liver inflammation by histological and transcriptomic analyses, nor the quantity of liver fibrosis. Livers from HFD mice had more myeloid populations, markedly fewer NKT cells and higher proportion of ST2+ Treg cells and ST2+ type 2 innate lymphoid cells (ILC2), all unaffected by IL-33 deficiency. In conclusion, deficiency of endogenous IL-33 does not affect the evolution of experimental diet-induced steatohepatitis towards liver fibrosis.
ISSN: 1949-2553

hal-01578584
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578584
DOI : 10.18632/oncotarget.18335
PUBMED : 28611297

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Comme dans d’autres régions du monde, la croissance démographique et l’accentuation des activités côtières au Canada entraînent une dégradation continue des écosystèmes et de la biodiversité. L’aménagement côtier contribue essentiellement à la vulnérabilité et à la détérioration des littoraux. Ce constat concerne le littoral acadien du Nouveau-Brunswick. Au-delà de ces pressions démographiques et de l’urbanisation sur les zones littorales, l'élévation du niveau marin et l'augmentation des températures (effets du changement et des variations climatiques) provoquent des impacts vérifiables : l’intensité des ondes de tempêtes, la dégradation des sols par l’érosion, etc. Ainsi, l’adaptation au changement climatique constitue un enjeu de taille pour la province du Nouveau-Brunswick. C’est pourquoi cet article propose d’étudier les impacts de ces changements auprès de deux régions côtières du littoral acadien et d’apprendre des expériences de chacun pour renforcer leur résilience. La recherche souhaite s’inscrire dans une nouvelle forme d’engagement communautaire qui prend en compte des aspects de la « gouvernance », de l’« aménagement du territoire » et de la « cohésion sociale ». L’article tente d’avoir une meilleure compréhension de l’enchâssement des aspects économiques et sociétaux dans les enjeux environnementaux.
As in other parts of the world, demographic growth and an increased use of coastal zones in Canada lead to a degradation of coastal ecosystems and a loss of biodiversity. Coastal development contributes in large part to the vulnerability and deterioration of coastal zones. This is very clearly seen in the case of the Acadian coast of New Brunswick. In addition to stresses from demographic growth and urbanization, the coastal zone is affected by sea level rise, temperature increases and other impacts of climate change such as increased climate variability, which lead to measurable effects such as an increase in storm intensity or increased coastal erosion. Thus, adaptation to climate change constitutes a major challenge for the province of New Brunswick. It is in this context that we propose to analyze the impacts of climate change in two sites on the Acadian coast and to profit from both experiences in order to increase the resilience of the communities through mutual learning. The research undertaken here aims at contributing to a new form of community engagement that takes “governance”, “land use planning” and “social cohesion” into account. The article aims at a better understanding of the interplay of social and economic aspects in this environmental problem.
Canada
Nouveau-Brunswick

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6970
http://journals.openedition.org/etudescaribeennes/6970

Éditeur(s) : Oxford university press
Résumé : The response of fish assemblages to changes in protection status is a major issue for both biodiversity conservation and fishery management. In New Caledonia, the Abore reef marine reserve harbours more than 500 fish species, and has been subjected to changes in protection status since 1988. The present study investigates the impact of these changes on a wide subset of species (213), based on underwater visual counts collected before the opening and after the closure to fishing of this marine protected area (MPA). We analysed the spatial and temporal variability in fish assemblage attributable to protection status, explicitly considering habitat. To understand the successive responses of fish assemblage to fishing and protection, the assessment models included four criteria de. ning species groups that partition the fish assemblage: trophic regime, adult size, mobility, and interest for fishing. We could therefore identify the negative impact of opening the MPA to fishing on piscivores and highly mobile species. Surprisingly, target species were not affected more than non-target species. Model results were used to identify species groups that respond to fishing and protection. These results utilize fisheries-related criteria to provide new insight into the response of fish assemblages to protection from the perspective of MPA monitoring.
ICES Journal of Marine Science (1054-3139) (Oxford university press), 2009 , Vol. 66 , N. 1 , P. 170-179

Droits : 2009 ICES/CIEM. Oxford Journals
http://archimer.ifremer.fr/doc/2009/publication-6130.pdf
DOI:10.1093/icesjms/fsn204
http://archimer.ifremer.fr/doc/00000/6130/

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Evaluer les retombées économiques de manifestations sportives sur les territoires d’accueil est l’une des conditions permettant de décider de la pérennisation ou non de ces évènements. Cette évaluation revêt une grande importance pour les prestataires de services, les institutionnels et les diverses entreprises qui y sont engagés, car elle leur permet d’avoir une vision plus claire du marché, et le cas échéant d’ajuster et d’affiner leur politique évènementielle pour l’avenir. Aujourd’hui, apparaît de plus en plus un besoin croissant d’évaluation de la part de praticiens et d’acteurs publics impliqués dans les activités sportives. Ces derniers comme le grand public s’interrogent quelquefois sur l’utilité ou la pertinence économique et sociale de tels évènements.Aussi, l’objectif prioritaire de cette étude est de proposer dans le cas de la Guadeloupe, à partir d’un cadre conceptuel précis et d’une méthodologie partagée et éprouvée, une évaluation territoriale de l’impact économique et social de la Route du rhum, en retenant deux approches: l’une en niveau, déterminant successivement les valeurs du multiplicateur input-output à l’aide d’un tableau entrées-sorties (TES) de l’économie guadeloupéenne, et du multiplicateur de la base qui s’appuie sur les résultats d’une méta-analyse; l’autre , dynamique, spécifie par un modèle économétrique l’influence de la Route du rhum et d’autres variables sur la croissance économique de la Guadeloupe. L’enjeu consiste à se doter collectivement de points de repère afin que les différents acteurs de ce sport puissent situer les effets de leurs actions par rapport à d’autres pratiques, d’autres territoires, et à clarifier le niveau de contribution économique de cette activité sportive sur le territoire de Guadeloupe. Les résultats obtenus convergent et montrent qu’en dépit d’un succès indéniable au plan sportif et médiatique, la Route du rhum, évènement sportif de voile de haut niveau reliant tous les quatre ans St Malo à Pointe-a-Pitre, enregistre au plan économique des résultats modestes si l’on en juge à la fois par les médiocres retombées induites en matière de fréquentation touristique, par les faibles coefficients multiplicateurs de revenu affichés, par un ratio coût-bénéfice défavorable et par les incidences mineures que provoque cet évènement sur la croissance économique régionale.
Evaluation of economic effects from sport events is necessary to pursue or not these events. This evaluation is important for enterprises that provide goods and services also for the government, because it allows them to obtain an accurate vision of the future.Today, we have a growing need of evaluation from the people involved in sport activities. Sometimes, these people ask themselves on economic and social advantages of these events.The objective of this paper is to propose for Guadeloupe a rigourous methodology that assesses the economic and social impact of Route du rhum which is a transatlantic competition between St-Malo and Pointe-a-Pitre. We use two approaches: the first of which is level-based and determines the values of both economic base multipliers and input-output multipliers by composing an input-output table of the Guadeloupe economy. The second, dynamic approach specifies from an econometric model the impact of several variables, especially those of la Route du rhum. The goal is to provide findings to stakeholders of this sport for comparison. The results of the investigation merge and show globally weak economic performance of Route du rhum, particularly a poor cost-benefit ratio, weak economic multipliers and inter-industry linkages.
Guadeloupe

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5296
http://journals.openedition.org/etudescaribeennes/5296

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Le changement climatique et le développement durable du tourisme représentent des enjeux majeurs pour l’avenir du tourisme dans plusieurs régions du monde, notamment aux Antilles françaises. Aussi, la présente étude se propose d’analyser et d’évaluer les effets économiques de la variation climatique sur le secteur touristique à la Guadeloupe et à la Martinique, pour quatre raisons essentielles : (a) le secteur du tourisme et les petites économies insulaires qui en dépendent, comme celles de la Guadeloupe et de la Martinique, sont fortement sensibles à la variabilité et au changement du climat. (b) la vulnérabilité intrinsèque de ces petites destinations et de leurs infrastructures à l’élévation du niveau de la mer et aux évènements climatiques extrêmes (cyclones, inondations, sécheresse), (c) la haute dépendance de l’industrie touristique régionale à l’utilisation des énergies fossiles, (d) enfin, en raison du fait qu’aucune évaluation économique chiffrée n’a été produite à ce jour sur les effets économiques de la la variation climatique sur le secteur touristique à la Guadeloupe et à la Martinique. Par conséquent, il importe de cerner dans ces deux régions les conséquences possibles de ce changement au plan touristique et d’envisager le cas échéant des stratégies d’adaptation et d’atténuation. Ce faisant, cette étude se fixe quatre objectifs principaux : (a) examiner les facteurs qui influencent la demande et l’offre de tourisme aux Antilles Françaises ; (b) effectuer à l’aide d’un modèle de demande touristique, l’impact économique de ces facteurs sur le nombre d’arrivées ainsi que sur la dépense touristique dans ces régions ; (c) évaluer et projeter en termes de coût, les effets du changement climatique sur l’activité touristique ; et (d) proposer aux autorités locales, des stratégies d’adaptation et d’atténuation face au défi du changement climatique.L’évaluation de ces différents effets est abordée ici à l’aide d’un modèle structurel de base (MSB) et d’un modèle structurel général (MSG), inspirés tous deux des travaux de Harvey (1990) pour capter à l’aide d’un indice climato-touristique, les éléments du climat qui impactent l’activité touristique. Cet indice synthétique est calculé en se basant sur les données climatiques du passé selon deux scénarios climatiques proposés par le groupe d’experts intergouvernemental sur l’évolution du climat (GIEC) – un scénario pessimiste A2, et un scénario optimiste B2-. Les résultats issus de l’application de ces modèles montrent que les caractéristiques climato-touristiques de la Guadeloupe et de la Martinique subiront vraisemblablement un déclin à long terme, et devraient au plan économique impacter négativement le secteur touristique de ces destinations, se traduisant par des pertes à la fois d’attractivité et de chiffre d’affaires du secteur. Les pertes subies par le tourisme en raison du changement climatique s’élèveraient à l’horizon 2100, et selon le scénario A2, à 45 millions d’euros à la Guadeloupe soit 0,7% de son PIB 2004, et à 60 millions d’euros à la Martinique soit 0,8% de son PIB 2004.
Climate change and the tourism sustainable development are important challenges for tourism future trends of many countries, including the French West Indies. Therefore, this study provides an evaluation of the likely economic effects of climate change on tourism sector in Guadeloupe and Martinique, and identifies four reasons why these countries should be concerned about the potential effects of climate change on tourism: (a) Guadeloupe and Martinique are island destinations very sensitive to climate change; (b) their intrinsic vulnerability and their infrastructure to sea level rise and extreme climatic events (e.g. hurricanes and floods); (c) the high dependence of their tourist industry on carbon-based fuels; and (d) up to date, no economic evaluation of climate change on tourism sector in Guadeloupe and Martinique is provided. Therefore, this study attempted to quantify the likely effects of the changes in the climatic factors on the economy of the French West Indies. There are four main objectives in this study. The first is to examine the factors that influence the demand and supply tourism in Guadeloupe and Martinique. The second is to forecast the impact of climate change to tourism arrivals until 2050 under two scenarios from the intergovernmental panel on climate change (IPCC) – A2: pessimistic scenario and B2: optimistic scenario-. Third is to evaluate and forecast the cost of climate change to the tourism sector until 2100. The fourth is to recommend to authorities the adaptation and mitigation strategies that can be undertaken by the tourism sector to French West Indies to address climate change.Following Harvey (1990), a Basic Structural Model (BSM) and a General structural Model (GSM), are employed to model tourist arrivals to Martinique. A tourism climatic index (TCI) conceptualized by Mieczkowski (1985) is used in this model to capture the elements of climate that impact on a destination’s experience. The results suggest that under both scenarios, the island’s key tourism climatic features will probably decline, and therefore, negatively impact on the destination experience of visitors. Tourism losses due to climate change are projected in 2100 to 45 millions euros in Guadeloupe, or 0.7% of its GDP, and to 60 millions euros in Martinique, or 0,8% of its GDP.
Guadeloupe
Martinique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6750
http://journals.openedition.org/etudescaribeennes/6750

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The Convective and Orographically-driven Precipitation Study (COPS) carried out in summer 2007 over northeastern France and southwestern Germany provided a fairly comprehensive description of the low-troposphere water-vapour field, thanks in particular to the deployment of two airborne differential absorption lidar systems. These lidar observations were assimilated using the 3D-Var assimilation system of the Application of Research to Operations at MEsoscale (AROME) numerical weather prediction mesoscale model. The assimilation was carried out for the period 4 July-3 August by running a three-hour forward intermittent assimilation cycle. First, the impact of the lidar observations was assessed by comparing the analyses with a set of more than 200 independent soundings. The lidar observations were found to have a positive impact on the analyses by reducing the dry bias in the first 500 m above ground level and by diminishing the root-mean-square error by roughly 15% in the first km. Then the impact of the lidar observations was assessed by comparing the precipitation forecasts (obtained with and without the lidar observations for the period 15 July-2 August) with the gridded precipitation observations provided by the Vienna Enhanced Resolution Analysis. In general, the impact was found to be positive but not significant for the 24 h precipitation and positive and significant for the 6 h precipitation, with an improvement lasting up to 24 h. Some selected case studies show that the improvement was obtained through a better depiction of convection initiation or through a more accurate positioning of the precipitation systems
ISSN: 0035-9009

hal-00659497
https://hal.archives-ouvertes.fr/hal-00659497
DOI : 10.1002/qj.1864

Éditeur(s) : Amer Geophysical Union
Résumé : Bolide impacts and large-scale volcanic eruptions have been proposed as possible causes of the massive extinction of life that has occurred episodically in Earth's history. In spite of the catastrophic disruption of the local environment that accompanies bolide impacts and volcanic eruptions, it has been difficult to explain why these events sometimes lead to global extinction of species. We propose that, in some cases, the missing link may be provided by hypercanes, runaway hurricanes that are capable of injecting massive amounts of water and aerosols into the middle and upper stratosphere, where they may have profound effects on atmospheric chemistry and radiative transfer. Hypercanes are theorized to occur when the sea surface temperature exceeds a critical threshold, which may occur when sea water is locally heated by bolide impact, shallow-sea volcanism, or possibly, by overturning of superheated brine pools formed by underwater volcanic activity. Simulations using a convection-resolving nonhydrostatic, axisymmetric numerical model show that hypercanes can indeed develop when the sea surface temperature is high, and that they inject large amounts of mass into the stratosphere.
Journal Of Geophysical Research-atmospheres (2169-897X) (Amer Geophysical Union), 1995-07 , Vol. 100 , N. D7 , P. 13755-13765

Droits : 1995 by the American Geophysical Union.
http://archimer.ifremer.fr/doc/00258/36966/35611.pdf
DOI:10.1029/95JD01368
http://archimer.ifremer.fr/doc/00258/36966/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Dudley et al. [9] commented on our paper [11], arguing that the current IUCN objective-based categorization ofprotected areas, which is also used in marine protected areas (MPAs), should not be abandoned and replaced bythe new regulation-based classification system [11]. Here we clarify that we do not advocate replacing thecurrent IUCN categories, but highlight the benefits of using both the objective-based IUCN categories and thenew regulation-based classification when applied to MPAs. With an increasing number of MPA types beingimplemented, most of them multiple-use areas zoned for various purposes, assessing ecological and socioeconomicbenefits is key for advancing conservation targets and policy objectives. Although the IUCN categoriescan be used both in terrestrial and marine systems, they were not designed to follow a gradient of impacts andthere is often a mismatch between stated objectives and implemented regulations. The new regulation-basedclassification system addresses these problems by linking impacts of activities in marine systems with MPA andzone classes in a simple and globally applicable way. Applying both the IUCN categories and the regulationbasedclasses will increase transparency when assessing marine conservation goals.
ISSN: 0308-597X

hal-01469541
https://hal-univ-perp.archives-ouvertes.fr/hal-01469541
DOI : 10.1016/j.marpol.2016.11.025

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : 9 pages
International audience
Potentially hazardous asteroids and comets have hit Earth throughout its history, with catastrophic consequences in the case of the Chicxulub impact. Here we reexamine one of the mechanisms that allow an impact to have a global effect—the release of climate-active gases from sedimentary rocks. We use the SOVA hydrocode and model ejected materials for a sufficient time after impact to quantify the volume of gases that reach high enough altitudes (> 25 km) to have global consequences. We vary impact angle, sediment thickness and porosity, water depth, and shock pressure for devolatilization and present the results in a dimensionless form so that the released gases can be estimated for any impact into a sedimentary target. Using new constraints on the Chicxulub impact angle and target composition, we estimate that 325 ± 130 Gt of sulfur and 425 ± 160 Gt CO2 were ejected and produced severe changes to the global climate.
ISSN: 0094-8276

hal-01639659
https://hal.archives-ouvertes.fr/hal-01639659
DOI : 10.1002/2017GL074879

Résumé : Publiée dans le cadre du premier Congrès international d'histoire coloniale à Paris
Siècle(s) traité(s) : 20
Droits : Domaine public
Provenance : Ville de Pointe-à-Pitre. Réseau des bibliothèques
Permalien : http://www.manioc.org/patrimon/PAP11103
PAP11103